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論文

Thermotolerant mutants of entomopathogenic fungi obtained by ion beam- and $$gamma$$ ray-induced mutagenesis

西東 力*; Fitriana, Y.*; 佐藤 勝也; 大野 豊; 鳴海 一成*

JAEA-Review 2014-050, JAEA Takasaki Annual Report 2013, P. 121, 2015/03

Entomopathogenic fungi, such as ${it Metarhizium anisopliae}$, are economically important agents for integrated pest management programmes of insect pests. However, negative effects of temperature, such as heat stress that exceeds 35 $$^{circ}$$C, can have serious deleterious effects on conidial germination and persistence, vegetative growth, sporulation, and/or the infection process in these entomopathogenic fungi. In this study, we attempted to enhance thermotolerance in a model entomopathogenic fungus, ${it M. anisopliae}$, by mutagenesis using ion beams or $$gamma$$ rays and evaluated the relative virulence of the resulting mutants compared with the wild-type isolates. In conclusion, ion beams and $$gamma$$ rays are useful tools for improving biological characteristics, such as thermotolerance and fungicide-tolerance, in entomopathogenic fungi, but the mutants obtained in this study must be carefully evaluated for unpredictable negative side effects.

論文

Benomyl-resistant ${it Beauveria bassiana}$ (Hypocreales: Clavicipitaceae) mutants induced by ion beams

Fitriana, Y.*; 篠原 忍*; 佐藤 勝也; 鳴海 一成*; 西東 力*

Applied Entomology and Zoology, 50(1), p.123 - 129, 2015/02

https://doi.org/10.1016/j.apsusc.2015.02.144
 被引用回数：0 パーセンタイル：1.75(Chemistry, Physical)

Fungicides targeted at phytopathogens can be harmful to entomopathogenic fungi. The purpose of this study was to use ion-beam irradiation technology to produce benomyl-resistant mutants of the entomopathogenic fungus ${it Beauveria bassiana}$. After irradiation of conidia at 150 Gy, two mutant isolates, BB22 and BB24, were selected on media containing the fungicide. In an assay of vegetative growth, BB22 and BB24 were over 500 and 800 times more tolerant to benomyl, respectively, compared with the wild isolate. A mutation was found at position 198 of the $$beta$$-tubulin gene in the mutant isolates, with a substitution of glutamate for alanine (E198A). Ion beams have great potential as a tool to improve the traits of entomopathogenic fungi such as increasing tolerance to fungicides. Fungicide-resistant mutants produced in this way could be useful agents for biological control within IPM programmes where fungicides are also used.

口頭

量子ビーム照射によって作出された昆虫病原糸状菌高温耐性変異体

西東 力*; Fitriana, Y.*; 佐藤 勝也; 鳴海 一成*

no journal, , 

化学合成農薬は数多くの課題を抱えていることから、天敵を利用する生物的防除法の開発が注目されている。害虫の生物的防除法の基幹技術のひとつとして昆虫病原糸状菌があるが、高温下において感染しにくいという難題を抱えている。本研究の目的は、炭素イオンビームあるいは$$gamma$$線の照射による突然変異育種技術を用いて、昆虫病原糸状菌(${it Metarhizium anisopliae}$)の高温耐性を強化することである。得られた高温耐性変異体については、植物病原糸状菌などにおいて高温耐性との関連が示唆されている遺伝子の変異を解析した。

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